Single Molecule Biophysics Contributed Talks MEASUREMENTS OF BIOMOLECULAR INTERACTIONS IN PHARMACEUTICAL & IMMUNODIAGNOSTIC SYSTEMS
نویسندگان
چکیده
Recent advances in the immunodiagnostics and pharmaceutical industries have demanded an improved understanding of the molecular interactions that underpin their technologies. For example, the design of more efficacious therapeutic agents requires a detailed knowledge of the proposed target biomolecule or biomolecular interaction. Techniques with the ability to probe single biomolecular interactions, such as the atomic force microscope (AFM), provide a new approach for the direct investigation of such processes at a fundamental level.
منابع مشابه
Dynamic fluctuations in single-molecule biophysics experiments: Comment on "Extracting physics of life at the molecular level: A review of single-molecule data analyses" by W. Colomb and S.K. Sarkar.
Single-molecule biophysics includes the study of isolated molecules and that of individual molecules within living cells. In both cases, dynamic fluctuations at the nanoscale play a critical role. Colomb and Sarkar emphasize how different noise sources affect the analysis of single molecule data [1]. Fluctuations in biomolecular systems arise from two very different mechanisms. On one hand ther...
متن کاملMeasuring and visualizing single molecular interactions in biology.
In recent years, considerable attention has focused upon the biological applications of the atomic force microscope (AFM), and in particular in its ability to explore biomolecular interaction events at the single molecule level. Such measurements can provide considerable advantages, as they remove the data averaging inherent in other biophysical/biochemical approaches that record measurements o...
متن کاملHigh-resolution, single-molecule measurements of biomolecular motion.
Many biologically important macromolecules undergo motions that are essential to their function. Biophysical techniques can now resolve the motions of single molecules down to the nanometer scale or even below, providing new insights into the mechanisms that drive molecular movements. This review outlines the principal approaches that have been used for high-resolution measurements of single-mo...
متن کاملMeasuring the forces that control protein interactions.
Although the force fields and interaction energies that control protein behavior can be inferred indirectly from equilibrium and kinetic measurements, recent developments have made it possible to quantify directly (a) the ranges, magnitudes, and time dependence of the interaction energies and forces between biological materials; (b) the mechanical properties of isolated proteins; and (c) the st...
متن کاملNew fluorescent tools for watching nanometer-scale conformational changes of single molecules.
Single-molecule biophysics has been serving biology for more than two decades. Fluorescence microscopy is one of the most commonly used tools to identify molecules of interest and to visualize biological events. Here we describe some of the most commonly used fluorescence imaging tools to measure nanoscale movements and the rotational dynamics of biomolecules.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 1999